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KMID : 0043319980210050576
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Archives of Pharmacal Research
1998 Volume.21 No. 5 p.576 ~ p.580
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Degradation of Acharan Sulfate and Heparin by Bacteroides stercoris HJ-15, a Human Intestinal Bacterium
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Kim Dong-Hyun
Kim Byung-Taek
Park Sun-Yong
Kim Na-Young
Han Myung-Joo
Shin Kuk-Hyun
Kim Wan-Suk
Kim Yeong-Shik
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Abstract
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When glycosaminoglycan (GAG)-degrating enzymes were measured in normal human stool suspensions, all 5 tested different stools degraded titrable heparin and acharan sulfate. GAG-degrading bacteria were screened from the isolates of human stools. Among them, HJ-15 had the most potent activities of heparinases (GAGs-degrading enzymes). However, HJ-15 produced the enzyme even if in the media without heparin. Acharan sulfate lyase was induced by acharan sulfate and heparin. Heparinase production was also induced by these GAGs. These enzymes, acharan sulfate lyase and heparinase, were produced in exponential and stationary phase of HJ-15 growth, respectively. Optimal pHs of the acharan sulfate lyase and heparinase activities were 7.2 and 7.5 respectively. the biochemical properties of HJ-15 was similar to those of B. stercoris. However, difference from B. stercoris was utilization of raffinose. this HJ-15 also degraded chondroitin sulfates A and C.
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KEYWORD
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Acharan sulfate, Heparin, Intestinal microflora, Acharan sulfate lyase, bacteroides stercoris HJ-15
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